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Figure 5 | EvoDevo

Figure 5

From: A cleavage clock regulates features of lineage-specific differentiation in the development of a basal branching metazoan, the ctenophore Mnemiopsis leidyi

Figure 5

Comb cells in cleavage-arrested embryos. The arrows point to the cilia of the differentiated comb cells. (A, B, C) DIC images of cleavage-arrested embryos. (A', B', C') Close-up views of the rectangles in (A, B, C). (D, E, F) DNA staining with Hoechst (blue) merged with DIC images of embryos inhibited with cytochalasin B at the 8-cell stage (D, E) or 16-cell stage (F). (G) Control embryo at 10 to 12 hpf. DIC images (H, I) and z-projections of confocal images (H', I') of embryos stained with anti-tyrosinated tubulin (red) showing the cilia, Alexa-488 phalloidin (green) showing cell borders and Hoechst 33342 (blue) showing the nuclei. hpf, hours post fertilization. Additional file3, Additional file4, Additional file5 are movies showing the motility of the cilia in the specimens shown in A, B and C respectively. Additional file6 shows the movement of the cilia in the comb rows of the specimen shown in G.

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ISSN: 2041-9139