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Fig. 12 | EvoDevo

Fig. 12

From: Expression of NK cluster genes in the onychophoran Euperipatoides rowelli: implications for the evolution of NK family genes in nephrozoans

Fig. 12

Expression of Tlx at consecutive developmental stages in embryos of the onychophoran E. rowelli. Anterior is left in A, C, and up in B, D, E; developing legs are numbered. A Stage III embryo in ventrolateral view. Note the two individual domains in the anterior limb buds (empty and filled arrowheads). Inset shows the same embryo stained with DAPI. B Detail of anterior limb buds of stage III embryo in ventral view. Note the ectodermal (arrows) and mesodermal domains (filled arrowheads) in the tips, and a separate proximal domain (empty arrowheads) in each limb bud. C Anterior end of a stage IV embryo in ventrolateral view. D Detail of the anterior limb buds of a stage IV embryo. Ventrolateral view. Note the ectodermal (arrows) and elongated mesodermal domains (arrowheads). E Developing fourth and fifth legs of a stage IV embryo in ventrolateral view. Note the ectodermal (arrows) and mesodermal domains (filled arrowheads) in the tips, and a separate proximal domain (empty arrowheads) in each limb bud. F Cross section of the developing fifth leg, dorsal is up. Note the ectodermal (arrow) and mesodermal domains (filled arrowhead) in the tips, and a separate proximal domain (empty arrowhead). F′ Same cross section as in F stained with DAPI. Developing nerve cord is indicated with dashed lines in F and F′. Abbreviations: at, developing antenna; cl, cephalic lobe; de, dorsal extra-embryonic tissue; jw, developing jaw; po, proctodeum; sp, developing slime papilla; st, stomodeum; ve, ventral extra-embryonic tissue. Scale bars: A: 500 µm; B, D, E, F, F′: 100 µm; C: 200 µm

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ISSN: 2041-9139