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Fig. 3 | EvoDevo

Fig. 3

From: Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development

Fig. 3

Development of aca3ˉaca2ˉ and aca1ˉaca3ˉaca2ˉ mutants. A Aggregation: Ppal aca3ˉaca2ˉ and aca1ˉaca3ˉaca2ˉ were starved on NN agar at 106 cells/cm2 on their own or mixed with 10% wild-type Ppal for the indicated time periods. Bar: 1 mm. B Fruiting bodies: the cells were developed into fruiting bodies, which were imaged in situ. Bars: 0.5 mm. C Spore and stalk cells: Ppal WT and aca1ˉaca3ˉaca2ˉ fruiting bodies were transferred to 0.001% Calcofluor and imaged under phase contrast (left) and epifluorescence (right). Bar: 20 µm

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