Fig. 3
Adapted from Schwager et al. 2017, with the addition of new Hox3-A expression data (Additional file 3: Fig. S9D). Ch cheliceral, Pp pedipalpal, L1-L4 leg-bearing 1 to 4, O1-O12 opisthosomal segments 1 to 12
Hox expression in scRNA-seq data. A Dotplot of the expression of all ten Hox markers in cell clusters ordered hierarchically for integrated data. B Temporal expression of thirteen Hox markers across the three stages. The legend in (B) relates to the expression and number of expressing cells per cluster in both (A) and (B). C Pearson’s correlation coefficients of SCT normalized average Hox expression across all 20 clusters reveal three cluster types related to pedipalpal, leg-bearing and opisthosomal identities. D Positioning of Hox expression across the A-P axis of P. tepidariorum. Spatial expression intensity (detected by RNA in situ hybridisation) reflected using opaque (strong expression) to more transparent colouring (weak expression).