Fig. 12
From: Shared regulatory function of non-genomic thyroid hormone signaling in echinoderm skeletogenesis
Immunohistochemistry using a monoclonal thyroxine antibody reveals localization of thyroxine positive cells at the tip of the developing archenteron and mouth. A Maximum Z-projection of gastrulating sea urchin embryo 40 hpf, showing thyroxine positive cells at the tip of the archenteron, A’ representative optical section in DIC showing some of the cells stained, A’’ negative control with pre-absorbed thyroxine antibody of stage-matched embryo shows no staining. B Maximum Z-projection of gastrulating sea urchin embryo 48.5 hpf, showing thyroxine positive cells at the tip of the archenteron and mouth. B’ representative optical section in DIC showing some of the cells stained, B’’ negative control with pre-absorbed thyroxine antibody of stage-matched embryo shows no staining. Scale bar: 10 μm. Pmc: Primary mesenchyme cells, bp: blastopore, a: archenteron, arrow points to positive staining