Fig. 8
From: Shared regulatory function of non-genomic thyroid hormone signaling in echinoderm skeletogenesis
MAPK and T4-binding site distribution in Pisaster ochraceus larva (brachiolaria) (A) Maximum intensity projection of a Pisaster ochraceus larva (brachiolaria) which has just begun rudiment development. Larvae were exposed for 90 min prior to fixation. B T4 increased MAPK phosphorylation in P.o. late bipinnaria/early brachiolaria relative to the control (n = 6, p < 0.05). RGD peptide decreased MAPK phosphorylation relative to the control (n = 6, p < 0.05). C Single slice of MAPK phosphorylation in midgut. C′ Single slice of T4-binding locations in midgut. D Coelom adjacent to gut. D′ MAPK phosphorylation in gut and coelom. D″ T4-binding locations in gut. Coelom shows little T4-binding. E Nuclear stain of hindgut. E′ MAPK phosphorylation in hindgut. E″ T4-binding locations in hindgut. Asterisk indicates significant difference from the control. hc: hydrocoel, gut: midgut, i: intestine leading to anus, sc: somatocoel