Fig. 3

DAPI stained images and stills from time-lapse images of stages 8 to 11. A Stage 8: perivitelline space (red arrowheads) forms at both poles of the embryo and allows embryo inversion to occur during which the embryo splits into two halves creating the ventral furrow. B Stage 9: the germband is fully extended and segmental grooves (white arrowheads) are visible. It is at this stage that true anterior and posterior ends are defined. (C, C′) Stage 10: lateral and dorsolateral views of the same embryo at Stage 10. During this stage, prosomal and opisthosomal segments are visible. Appendages primordia begin to form. (D, D′) Stage 11: germband retraction begins and appendages start to elongate. A—anterior; P—posterior; L1–L4—Budding legs 1–4; O1–O4—opisthosomal segments; PCL—pre-cheliceral lobe, pp—budding pedipalps; lb—labium; Hd—head region; V—ventral furrow/sulcus; GZ—growth zone (post-abdomen. Scale bars are 100 µm. st: stage