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Fig. 1 | EvoDevo

Fig. 1

From: Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata

Fig. 1

Astrangia poculata is a tractable research organism for functional molecular studies in corals. A A. poculata colony showing extended polyps. B A. poculata colony during spawning; arrows point to sperm emerging from two polyps. C, D Zygotes resting on nylon mesh fixed in the bottom of a 35-mm petri dish in preparation for microinjection. E Live image of a 16-cell stage embryo injected at the 2-cell stage with two different dextran dyes (dex-488, dex-555). F Injection of mRNA encoding a TCF-venus fusion protein from Nematostella vectensis (NvTCF::venus) demonstrates proper translation and nuclear localization of exogenous mRNAs; arrow points to cells in M-phase. Scale bars = 20 µm

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ISSN: 2041-9139